Given their geographic situation, landlocked developing countries have been hit doubly hard by the cross-border processes implemented to contain the coronavirus disease (COVID-19) pandemic. This research provides a detailed explanation of the responses to the COVID-19 pandemic in the areas of international trade and transport adopted by the Governments of the Plurinational State of Bolivia and of Paraguay to limit the effects of the pandemic on trade operations and supply chains in their countries. It also provides a set of policy recommendations to preserve and improve trade facilitation and transport connectivity with transit countries in the framework of Vienna Programme of Action for Landlocked Developing Countries for the Decade 2014–2024.